滬鼎產品文獻:人IL-6,TNF-α,iNOS ELISA試劑盒引用文獻

【文獻標題】Rapeseed protein-derived ACE inhibitory peptides LY, RALP and GHS show

antioxidant and anti-inflammatory effects on spontaneously hypertensive rats

【作者】Rong He，Yujiao Wang，Yijie Yang，et.al

【作者單位】南京財經大學（Nanjing University of Finance and Economics）

【文獻中引用產品】

人白細胞介素6(IL-6)ELISA試劑盒

人腫瘤壞死因子α(TNF-α)ELISA試劑盒

人誘導型一氧化氮合成酶(iNOS)ELISA試劑盒

【關鍵詞】Rapeseed peptide，ACE inhibition，Anti-inflammatory effect，Cytokine，Oxidative  stress

【DOI】doi.org/10.1016/j.jff.2019.02.031

【影響因子(IF)】5.9

【出版期刊】《Journal of Functional Foods》

【產品原文引用】

Materials and reagents

RAW264.7 cells were obtained from the cell bank of the Chinese Academy of Sciences(Shanghai,China). LPS from Escherichia coli O55:B5,3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide(MTT), fetal bovine serum, and hematoxylin and eosin (H&E) solutions were purchased from Sigma-Aldrich Trading Co., Ltd. (Shanghai,China). The SOD Assay Kit, Malondialdehyde (MDA) Assay Kit, Nitric Oxide (NO) Assay Kit and Nitric Oxide Synthase (NOS) Assay Kit were purchased from Jiancheng Bioengineering Institute (Nanjing, China).The Human Interleukin 6 (IL-6) ELISA Kit, Tumor Necrosis Factor-α(TNF-α) ELISA Kit and Inducible Nitric Oxide Synthase (iNOS) Assay Kit were purchased from Shanghai HuDing Biological Technology Co., Ltd.(Shanghai, China). Phosphate-buffered saline (PBS, pH 7.4) was purchased from Solarbio Science and Technology Co., Ltd. (Beijing, China).All other chemical reagents were of analytical grade. The ACE inhibitory peptides Leu-Tyr (LY), Arg-Ala-Leu-Pro (RALP) and Gly-His-Ser(GHS) were synthesized with purity over 95% by Synthgene Biological Technology Co., Ltd. (Shanghai, China).

滬鼎產品文獻:人Tau蛋白(TAU)ELISA試劑盒引用文獻

【文獻標題】Effect of erythropoietin combined with hypothermia on serum tau protein levels and neurodevelopmental outcome in neonates with hypoxic-ischemic encephalopathy

【作者】Hong-yan Lv，Su-jing Wu，Qiu-li Wang，et.al

【作者單位】邯鄲市邯鄲婦幼保健院（Handan Maternal and Child Health Care Hospital, Handan）

【文獻中引用產品】

人Tau蛋白(TAU)ELISA試劑盒

【關鍵詞】nerve regeneration; erythropoietin; hypothermia; hypoxic-ischemic encephalopathy; neonate; tau protein; biomarkers; prognosis; neuroprotection; neural regeneration

【DOI】doi.org/10.4103/1673-5374.217338

【影響因子(IF)】3.6

【出版期刊】《NEURAL REGENERATION RESEARCH》

【產品原文引用】

Serum tau protein measurement

Blood samples were taken from the radial vein at 24 hours after birth (before EPO treatment) and 4, 8, and 12 days after EPO treatment. Blood samples were placed in a test tube (specifications: 5 mL, No A06277602; Hebi City Zhong Xing Medical Supplies, Hebi, China), maintained without stirring for 30 minutes at room temperature, and then centrifuged in a L-400 low-speed automatic balancing centrifuge (Hunan Xiangyi Laboratory Instrument Development, Changsha, Hunan Province, China) at 3,000 r/min for 15 minutes.Serum was removed into another test tube and placed in a freezer at ?70°C until use. To avoid any influence on the results, blood transfusions to the neonates were avoided before collecting blood samples. Serum tau protein was detected using an enzyme-linked immunosorbent assay. The kit was provided by the Shanghai HuDing Biological Science and Technology Co., Ltd.

滬鼎產品文獻:人白介素4,6,8,10,HGF,TIMP-1等ELISA試劑盒引用文獻

【文獻標題】水凝膠三維培養對人羊膜間充質干細胞特性及旁分泌效應的影響

【作者】王 旗，楊曉雙，王達利

【作者單位】遵義醫科大學

【文獻中引用產品】

人白細胞介素4(IL-4)ELISA試劑盒

人白細胞介素6(IL-6)ELISA試劑盒

人白細胞介素8(IL-8)ELISA試劑盒

人白細胞介素10(IL-10)ELISA試劑盒

人肝細胞生長因子(HGF)ELISA試劑盒

人金屬蛋白酶組織抑制因子-1(TIMP-1)ELISA試劑盒

人基質金屬蛋白酶1(MMP-1)ELISA檢測試劑盒

人表皮生長因子（EGF）ELISA試劑盒

人轉化生長因子β1(TGF-β1)ELISA試劑盒

人堿性成纖維細胞生長因子(bFGF)ELISA試劑盒

人角化細胞生長因子(KGF)ELISA試劑盒

人血管內皮生長因子(VEGF)ELISA試劑盒

【關鍵詞】水凝膠；羊膜間充質干細胞；二維培養；三維培養；創面修復；旁分泌效應

【DOI】10.3969/j.issn.2095-4344.2299 

【影響因子】1.1

【出版期刊】《中國組織工程研究》

【產品原文引用】

ELISA法檢測 :

ELISA法檢測兩種培養條件下hAMSCs旁分泌創面愈合相關因子的蛋白表達情況。將hAMSCs按照2×104

/cm2接種于兩種不同培養條件的24孔板上。根據二維培養的細胞情況，細胞貼壁后用PBS清洗兩種培養條件下的hAMSCs并更換新鮮培養基，放入細胞培養箱繼續培養48 h。48 h后收集兩種細胞培養液， 1 000×g離心20 min，取上清。根據Elisa試劑盒(上海滬鼎)說明書進行加樣，在450 nm波長處測定各孔的吸光度值。繪制標準曲線，計算白細胞介素4、白細胞介素6、白細胞介素8、白細胞介素10、肝細胞生長因子、金屬蛋白酶組織抑制因子、基質金屬蛋白酶、表皮生長因子、轉化生長因子β1、堿性成纖維細胞因子、角化細胞生長因子、血管內皮生長因子的相應濃度。

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