蘇州阿爾法生物實驗器材有限公司
蘇州阿爾法生物實驗器材有限公司
細胞名稱：EMT-6細胞-小鼠乳腺癌細胞 細胞別稱：EMT-6;EMT6細胞,Experimental Mammary Tumour-6貨號：TCM-C728規格：1X10^6/ t25細胞培養瓶價格：目錄價1500元(干冰運輸需另加200元干冰費)EMT-6細胞培養基貨號：TCM-G728
EMT-6細胞-小鼠乳腺癌細胞
細胞名稱：EMT-6細胞-小鼠乳腺癌細胞
細胞別稱： EMT-6; EMT6細胞,Experimental Mammary Tumour-6
貨號：TCM-C728
規格： 1X10^6/ t25細胞培養瓶
價格：目錄價1500元 (干冰運輸需另加200元干冰費)
細胞簡稱：emt6細胞
產品貨號：TCM-C728
種屬來源：小鼠
組織來源：乳腺
特征：乳腺癌
細胞形態：上皮細胞樣
生長特性：貼壁生長
培養體系：Waymouth's MB 752/1+15%FBS+1%Glutamax+1%P/S
EMT-6細胞-小鼠乳腺癌細胞 配套培養基貨號：TCM-G728
傳代比例：1:4-1:8，每2-3天換液一次
傳代周期：24-48 h
培養條件：氣相：95%空氣+5%CO2，溫度：37℃
凍存條件：60%基礎培養基+30%FBS+10%DMSO，液氮儲存
質量檢測：細菌、真菌、支原體檢測均為陰性
EMT-6是在BALB/cCRGL小鼠的增生性乳腺泡結節植入后發生的可移植小鼠乳腺癌中建立的。所得到的腫瘤系(命名為KHJJ)在BALB/cKa小鼠中繁殖，動物傳代25次后適應組織培養，命名為EMT。EMT6是1971年在斯坦福大學分離的EMT克隆分離株。
"Rockwell SC, et al. Characteristics of a serially transplanted mouse mammary tumor and its tissue-culture-adapted derivative. J. Natl. Cancer Inst. 49: 735-749, 1972. PubMed: 4647494
Palom Y, et al. Structure of adduct X, the last unknown of the six major DNA adducts of mitomycin C formed in EMT6 mouse mammary tumor cells. Chem. Res. Toxicol. 13: 479-488, 2000. PubMed:
Collingridge DR, Rockwell S. Pentoxifylline improves the oxygenation and radiation response of BA1112 rat rhabdomyosarcomas and EMT6 mouse mammary carcinomas. Int. J. Cancer 90: 256-264, 2000. PubMed:
Rockwell S, Kelley M. RSR13, a synthetic allosteric modifier of hemoglobin, as an adjunct to radiotherapy: preliminary studies with EMT6 cells and tumors and normal tissues in mice. Radiat. Oncol. Investig. 6: 199-208, 1998. PubMed: 9822166
Rockwell S. In vivo-in vitro tumor systems: new models for studying the response of tumors to therapy. Lab. Anim. Sci. 27: 831-851, 1977. PubMed: 338981
Rockwell STumor-Cell SurvivalIn: Rockwell STumor models in cancer researchTotowa, New JerseyHumana Press Inc.617-631.
EMT6 was established from a transplantable murine mammary carcinoma that arose in a BALB/cCRGL mouse after implantation of a hyperplastic mammary alveolar nodule. The resulting tumor line (named KHJJ) was propagated in BALB/cKa mice and adapted to tissue culture after the 25th animal passage, and the cell line was named EMT. EMT6 is a clonal isolate of EMT isolated in 1971 at Stanford University. The EMT6 cell line can be grown either in animals as a tumor or in tissue culture. Cells derived from tumors have a reported in vitro plating efficiency of 30%. Cell grown in tissue culture reportedly have a plating efficiency of 70%.
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