功能大、操作、發表文獻最多的葉綠素熒光成像系統
突變株快速篩選的強大工具
? 標準藍光版，450 nm，測葉片和真核藻類 ? 紅光版，620 nm，測藍藻和真核藻類 |
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主要功能
? 成像功能：對Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red等參數進行成像分析。測定調節性能量耗散Y(NPQ)，反映植物光保護能力，測定非調節性能量耗散Y(NO)，反映植物光損傷程度。
? 程序測量功能：可程序測量熒光誘導曲線、快速光曲線和暗弛豫，也可手動測量；在測量過程中能自動分析所有熒光參數的變化趨勢
? AOI功能：可在測量前或測量后任意選擇感興趣的區域（AOI），程序將自動對選擇的AOI的數據進行變化趨勢分析，并在報告文件中顯示相關AOI的數據。所有報告文件中顯示的數據都可導出到EXCEL文件中。
? 成像異質性分析功能：對任意參數任意時間的成像，可在圖像上任意選取兩點，軟件自動對兩點間的數據進行橫向異質性分析，并可導出到EXCEL文件中。
? 成像數據范圍分析功能：對任意參數任意時間的成像，可分析任意兩個熒光數值之間有多少個像素點，多少面積（cm2）。
? 突變株篩選功能：可跟據成像結果快速篩選光合、產氫/油、抗逆（抗鹽、抗旱、抗病等）等突變株。
? 微藻毒理研究功能：可同時測量96個微藻樣品（對照和處理組）的光合活性，軟件自動給出處理組樣品相對于對照組的光合抑制百分比。
? 吸光系數測量功能：快速測量葉片的吸光系數。吸光系數測量光源： 16個紅光（650 nm）和16個近紅外（780 nm）LED，用于測量植物葉片或藻類樣品PAR吸光系數。
? 監測功能：可實時監控箱內環境狀態、機器狀態監控區顯示目前程序及環境狀態
? 環境仿真功能：可程序化多組程序功能 (最多6組)，可個別設定執行天數，可設備單日環境參數（最小間隔為分），程序化環境參數
? 項目管理功能：預約存多次測量的植物影像及參數，延時（縮時）攝影功能
? 數據分析功能：可導出照片文件，可導出葉綠素熒光參數Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red等
系統設計
? 可單獨作為葉綠素熒光儀使用，方便帶到野外田間測量和成像；也可配置箱體式結構，容量300L，能同時滿足植物培養和葉綠素熒光成像的功能。箱體內設有栽培區，可分2層、3層、4層等，調節高度進行培養和測量。箱體內有特殊消光圖層，避免反光造成的測量干擾。
? 系統配置觸摸式高清顯示屏（24寸），方便操作，支持1670萬色彩和FHD高清畫質。
? 系統處理器：I7處理器，16G緩存，512固態硬盤SSD，滿足快速數據處理和存儲要求。
? 供電：室內使用220V，50Hz交流電，熒光儀單獨使用時，內置可充電鋰電池供電，滿足4小時的野外測量
箱體環境控制（光照、溫度等）
? 溫度環控條件：15-40°C ±5°C（關燈）
? 光照系統：采用四合一LED光盤，白光5000k ±500k、紅光660nm±10nm、藍450nm±10nm、紅外光730nm±10nm，光盤面積29×40cm，四種光源可獨立自主控制，光質強度PPFD ≧700µmols-1m-2@15cm，光照時間控制可程序化設定，最小設定時間1min，可仿真日出至日落光照變化。
? 控制系統：PID微電腦控制，可設定上晝夜循環程序
? 可選配二氧化碳和濕度控制單元
成像技術參數
Maxi探頭
? 熒光測量光源： 44個藍色LED，450 nm，測量光強度0.5 μmol m-2 s-1PAR，光化光強度1900 μmol m-2 s-1PAR，飽和脈沖強度4000 μmol m-2 s-1PAR
? 吸光系數測量光源：16個紅光（660 nm）和16個近紅外（780 nm）LED，用于測量樣品PAR吸光系數。
? 成像面積：工作距離18.5cm，成像面積 10×13 cm；工作距離22.5cm，成像面積11×15cm。
? 光強異質性：測量區域光強異質性小于±7％。
? 測量參數：Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red等。
應用領域
? 光合作用研究：可以在相同的條件下同時對大量樣品進行成像
? 植物病理學：病斑部位（包括肉眼不可見時）成像以及病斑擴散的時空動力學
? 植物脅迫生 理學：肉眼不可見脅迫損傷的早期檢測
? 遺傳育種：出苗后大規模快速篩選高光合/抗旱/抗熱/抗凍/抗病等植株
? 突變株篩選：快速篩選模式植物的光合突變株、抗逆突變株、產氫微藻突變株等
? 微藻毒理學：不同毒物濃度多個重復的樣品一次測完，軟件自動計算抑制比率
? 分子生物學：宏觀水平上檢測樣品的綠色熒光蛋白（GFP）熒光
? 其它多種擴展研究
成像參數
Fo, Fm, F, Ft, Fm', Fv/Fm, Y(II), qL, qP, qN, NPQ, Y(NPQ), Y(NO), ETR, Abs, NIR和Red等
突變株的快速篩選
MAXI-IMAGING-PAM特別適合對幼苗、愈傷組織、微藻等進行突變株的快速篩選，適合于與光合突變株、抗逆（抗旱、抗鹽、抗病等）突變株、產油/氫突變株等的快速篩選。
國外利用MAXI-IMAGING-PAM篩選突變株的典型客戶如拜耳、BASF、孟山都、先正達等大型跨國農業，以及各大農業育種、植物分子生物學等科研單位，例如澳大利亞植物功能基因組中心（阿德雷德大學）、德國尤里希表型植物表型研究中心（Julich Plant Phenotyping Centre）等等。
國內約一半的MAXI-IMAGING-PAM客戶在進行突變株快速篩選工作，主要分布于、中國農科院和各大高校。
突變株篩選實例一：國內某客戶篩選的擬南芥突變株
突變株篩選實例二：產油突變株的篩選。Ajjawi et al, 2010, Plant Physiol., 152: 529-540.
突變株篩選實例三：光合突變株的篩選。Armbruster et al., 2010, Plant Cell, 22: 3439-3460.
調制葉綠素熒光成像實例
葉片成像異質性 1）葡萄葉片
2）荷花葉片
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水果的成像 1）草莓的成像
2）獼猴桃的成像
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突變株篩選
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植物病理研究
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熒光成像與CO2氣體交換的同步測量
（GFS-3000/IM-MAXI）：MAXI-探頭與GFS-3000聯用，在10 cm x 13 cm的面積上同步測量氣體交換與熒光成像。
產地：德國WALZ
代表文獻
數據來源：光合作用文獻Endnote數據庫
原始數據來源：Google Scholar
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